[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 27, Issue 4 (10-2015) ::
J Iran Dent Assoc 2015, 27(4): 203-208 Back to browse issues page
A Comparative Study of Immunohistochemical Expression of BAX Protein in Radicular Cyst and Keratocystic Odontogenic Tumor
Shima Nafarzadeh1, Ahmad Reza Sarrafan *2, Hamid Abbaszadeh Bidokhti3, Hemat Gholinia4
1- Assistant Professor, Department of Oral and Maxillofacial Pathology, School of Dentistry, Babul University of Medical Sciences, Babul, Iran
2- Dental Student, Member of Research Committee, School of Dentistry, Babul University of Medical Sciences, Babul, Iran , a.sarrafan@mubabol.ac.ir
3- Member of Dental Material Research Center, School of Dentistry, Babul University of Medical Sciences, Babul, Iran
4- Health Research Institute, Babul University of Medical Sciences, Babul, Iran
Abstract:   (2428 Views)

Background and Aim: Keratocystic odontogenic tumor (KCOT), formerly known as odontogenic keratocyst (OKC), is a developmental odontogenic cyst with an invasivebehavior and high recurrence rate. Radicular cyst (RC) is an inflammatory non-invasive dental cyst, with no tendency to recurrence. Bcl2-associated X (BAX) protein acts as an apoptotic activator (pro-apoptotic protein), disrupts the integrity of mitochondrial membrane, and leads to the loss of outer membrane and release of cytochrome c from the inner membrane into the cytosol. Since KCOT is more aggressive and has a greater tendency to recurrence than RC, the aim of this study was to assess the immunohistochemical expression of BAX protein in the epithelial lining of RC and KCOT.

Materials and Methods: In this descriptive analytical study, paraffinized blocks of 17 RCs and 17 KCOTs fixed in 10% formalin were used (all KCOT samples were non-inflammatory and non-syndromic). After confirming the diagnoses by reviewing the slides, tissuespecimens with sufficient length of epithelium were stained by immunohistochemistry (IHC) for BAX marker. Then, the stained cells were randomly counted in five high power fields. Analysis of the data was performed using SPSS 21 via Student's t-test and chi-test.

Results: Percentages of staining for BAX marker were 16.4±9.4% and 66.4±12.3% in the epithelial lining of KCOT and RC, respectively and there was a significant difference between them (P<0.001).

Conclusion: According to the findings, lower expression of BAX protein in KCOT justifies its more aggressive behavior and tendency to recurrence compared to RC.

Keywords: BAX protein, human, Odontogenic Cysts, Radicular Cyst, Immunohistochemistry
Full-Text [PDF 885 kb]   (1193 Downloads)    
Type of Study: Orginal | Subject: Patology
References
1. Oda D, Rivera V, Ghanee N, Kenny E, Dawson K. Odontogenic keratocyst: The northwestern USA experience. J Contemp Dent Pract. 2000 Feb; 1(2): 60-74.
2. Reichart PA, Philipsen HP, Sciubba JJ. The new classification of head and neck tumours (WHO)-any changes? Oral Oncol. 2006 Sept;42(8):757-8.
3. Soluk Tekkeşın M, Mutlu S, Olgaç V. Expressions of bax, bcl-2 and Ki-67 in odontogenic keratocysts (keratocystic odonto-genic tumor) in comparison with ameloblasto-mas and radicular cysts. Turk Patoloji Derg. 2012;28(1):49-55.
4. Neville BW, Damm DD, Allen CM, Bouquot JE. Oral & Maxillofacial Pathology. 3rded. Philadelphia: Saunders; 2009. Chapter:15.
5. Regezzi JA, Sciubba JJ, Jordan RCK: Oral Pathology: Clinical Pathologic Correlations. 5th ed. Philadelphia, Elsevier Saunders; 2008, 245.
6. Rangiani A, Motahhary P. Evaluation of bax and bcl-2 expression in odontogenic keratocysts and orthokeratinized odontogenic cysts: A comparison of two cysts. Oral Oncol. 2009 Jul;45(7):e41-e44.
7. Billen LP, Kokoski CL, Lovell JF, Leber B, Andrews DW. Bcl-XL inhibits membrane permeabilization by competing with Bax. PLoS Biol. 2008 Jun;6(6):e147.
8. Baliga BC, Kumar S. Role of bcl-2 family of proteins in malignancy. Hematol Oncol. 2002 Jun; 20(2):63-74.
9. Krajewski S, Hugger A, Krajewska M, Reed JC, Mai JK. Development expression patterns of bcl-2, bcl-X, bax and bak inteeth. Cell Death Differ. 1998 May; 5(5):408-415.
10. Shear M. The aggressive nature of the odontogenic keratocyst: is it a benign cystic neoplasm? Part 2. Proliferation and genetic studies. Oral Oncol. 2002 Jun; 38(4):323-331.
11. Zhang M, Zhang P, Zhang C, Sun J, Wang L, Li J, et al. Prognostic significance of Bcl2 and Bax protein expression in the patients with oral squamous cell carcinoma. J Oral Pathol Med. 2009 Mar; 38(3):307-13.
12. Kumar V, Abbas AK, Aster JC. Robbins basic pathology: Elsevier Health Sciences; 2012.
13. Jahanshahi Gh, Talebi A, Shirvani A. Expression of Bcl-2 in the epithelial lining of odontogenic keratocysts. J of Dent Tehran Univ of Med Sci. 2006 Nov; 3(1):30-35.
14. Saghravanian N, Habibi A, Mohtasham N, Afzal Aghaie M, Shiva A, Babazadeh S. Evaluation of MDM2 and P53 expression in dentigerous, radicular and residual cysts by immunuhistochemistry. J of Mashhad Dent Sch. 2009 May;33(2):145-52.
15. Piatelli A, Fomni M, Rubini C. Differentiation of odontogenic keratocyst from other odonto-genic cysts by the expression of BCL 2 immunoreactivity. Oral Oncol. 1998 Sept; 34(5): 404-407.
16. Narain P, Parihar S, Verma A, Sharma A. Differentiation of ameloblastoma, odontogenic keratocyst and dentigerous cysts by BCL-2 Protein-an immunohistochemical study. Indian J of Contemp Dent. 2015 Jul; 3(2):54-9.
17. Razavi SM, Torabinia N, Mohajeri MR, Shahriyary S, Ghalegolab S, Nouri S. Expres-sion of Bcl-2 and epithelial growth factor recep-tor proteins in keratocystic odontogenic tumor in comparison with dentigerous cyst and ameloblastoma. Dent Res J (Isfahan). 2015 Jul-Aug; 12(4):342-7.
18. Kolář Z, Geierová M, Bouchal J, Pazdera J, Zbořil V, Tvrdý P. Immunohistochemical analysis of the biological potential of odontogenic keratocysts. J Oral Pathol Med. 2006 Feb;35(2):75-80.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nafarzadeh S, Sarrafan A R, Abbaszadeh Bidokhti H, Gholinia H. A Comparative Study of Immunohistochemical Expression of BAX Protein in Radicular Cyst and Keratocystic Odontogenic Tumor. J Iran Dent Assoc 2015; 27 (4) :203-208
URL: http://jida.ir/article-1-1818-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 27, Issue 4 (10-2015) Back to browse issues page
Journal of Iranian Dental Association

AWT IMAGE

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4509