[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
Main Menu
Journal Information::
Editorial Policies::
Articles archive::
For Authors::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Last site contents
:: Contact Us
:: Volume 18, Issue 2 (Foll Text in Persian 2006) ::
J Iran Dent Assoc 2006, 18(2): 72-80 Back to browse issues page
In vitro biocompatibility of eight orthodontic materials in human oral fibroblasts
Sosan Sadeghian * 1, Mohammad hosein Nasr Esfahani , Fariba Molavi
1- , drsadeghian@yahoo.com
Abstract:   (21482 Views)

In vitro biocompatibility of eight orthodontic materials in human oral fibroblasts

Dr. S. Sadeghian* - Dr. MH. Nasr Esfahani** - F. Molavi***

 *- Assistant Professor of Orthodontics Dept. - Faculty of Dentistry – Islamic Azad University (Khorasgan).

**- Associate Professor of Embryology, Royan Institute,

***- Member of Royan Institute,

Background and Aim: In orthodontic therapy, different materials are used and subjected to a damp oral environment, which can modify their properties. The purpose of this study was to evaluate the biocompatibility of eight metallic and non metallic orthodontic materials in human oral fibroblast culture.

 Materials and Methods: The specimens consisted of eight clinically used materials, including two coil springs, two glass ionomer cements, two O-rings and two orthodontic acrylic resins. Teflon was used as a negative control. After a release period of each material in the culture medium for 20 days, the viability of oral cultivated fibroblasts was compared to the negative control by MTT assay. Biocompatibility was also assessed using the direct method in which cell viability was evaluated by an invert phase contrast microscope, after direct exposure to the tested materials.

Results: MTT assay showed stainless steel (P.V=0.665) and Bayer acrylic resin (P.V=0.179) to be biocompatible with gingival fibroblasts. Significant differences were observed between the biocompatibility of all study groups [Nickel titanium (PV=0.037), Acropars acrylic resin (PV=0.014) and both studied O-rings (PV=0.001), (PV=0.016)] and the control group. According to the direct method, stainless steel and Nickel titanium showed grade 1, glass ionomer cements grade 4 and other materials revealed grade 0 cytotoxicity.

 Conclusion: The two studied glass ionomer cements were highly cytotoxic. Bayer acrylic resin was more biocompatible than Acropars acrylic resin.

Key words: Biocompatibility - Human oral fibroblasts - Orthodontic materials - MTT assay

Keywords: Biocompatibility, Human oral fibroblasts, Orthodontic materials, MTT assay
Full-Text [PDF 271 kb]   (2740 Downloads)    
Type of Study: Research | Subject: General
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadeghian S, Nasr Esfahani M H, Molavi F. In vitro biocompatibility of eight orthodontic materials in human oral fibroblasts. J Iran Dent Assoc 2006; 18 (2) :72-80
URL: http://jida.ir/article-1-56-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 2 (Foll Text in Persian 2006) Back to browse issues page
Journal of Iranian Dental Association

AWT IMAGE

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Persian site map - English site map - Created in 0.04 seconds with 37 queries by YEKTAWEB 4645