:: Volume 24, Issue 3 (7-2012) ::
J Iran Dent Assoc 2012, 24(3): 86-94 Back to browse issues page
Tissue Engineering and Stem Cells in Periodontal Regeneration
Ardeshir Lafzi1, Reza Amid *2, Mehdi Kadkhodazadeh3, Kimia Rohani4, Sahar Haddadpoor4, Liela Safiyari4, Sara Saedi4
1- Professor, Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences. Tehran, Iran
2- Assistant Professor, Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences. Tehran, Iran , reza_amid@yahoo.com
3- Assistant Professor, Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences. Tehran, Iran
4- Dental Student, School of Dentistry, Shahid Beheshti University of Medical Sciences. Tehran, Iran
Abstract:   (11513 Views)
   Background and Aim : Reconstructive treatments in dentistry aimed at achieving complete regeneration of destroyed structures both in soft and hard tissues. To date, this goal has been tried to accomplish using various bone grafts, growth factors, and barrier membranes. Stem cells are the most fascinating area of biology today and have been used clinically in the field of medicine to treat many incurable diseases . 

  Materials and Methods : The English literature indexed in in the MEDLINE/PubMed database was systematically searched and original papers were critically reviewed on this subject, until the second quarter of 2011. Additional papers were obtained by manual searching reference lists of previously published review papers .

  Results : More than fifty years ago, the first allogenic hematopoietic stem cell was transplanted in patients. However, the promise of other stem cell populations for tissue replacement and repair remains unachieved. When considering cell-based interventions for tissue regeneration, the factors influencing therapeutic success and safety are more complicated than for traditional treatments. So, it is important for dentists to have a brief understanding about the principles and clinical applications of stem cells in tissue engineering field .

  Conclusion : This article reviews the most recent published data to regenerate dental and periodontal tissues based on scientific principles and provides fundamental information to readers about the different aspects involved in tissue engineering. Ideal combination of cells, scaffolds, and growth factors for tissue engineering may be extended over future years. The findings collected in this literature review show that we are now at a stage in which engineering a complex tissue, such as the periodontium, is no longer an unachievable goal and the next decade will certainly be an exciting time for dental and periodontal research .






Keywords: Periodontal regeneration, Stem cells, Literature review
Full-Text [PDF 389 kb]   (2708 Downloads)    
Type of Study: Orginal | Subject: Periodontology
1. Langer R, Vicanti JP .Tissue engineering. Sci-ence 1993 May 14;260(5110):920-6.
2. Gyorevski N, Nelson CM. Bidirectional ex-tracellular matrix signaling during tissue mor-phogenesis. Cytokine & Growth Factor Reviews 2009 Oct-Dec;20(5-6):459-65.
3. Mimeault M, Hauke R, Batra SK. Stem cells: A revolution in therapeutics-recent advances in stem cell biology and their therapeutic applica-tions in regenerative medicine and cancer thera-pies. Clin Pharma & Therap. 2007 Sep; 82(3): 252-64.
4. Allison MR, Islam S. Attributes of adult stem cells. J Pathol. 2009 Jan; 217(2):144-60.
5. Kiourac DC, Ito C, Csaszar E, Roch A, Yu M, Sykes EA, et al. Dynamic interaction networks in a hierarchically organized tissue. Mol Syst Biol. 2010 Oct;6(5):417-33.
6. Daley WP, Peters SB, Larsen M. Extracellular matrix dynamics in development and regenera-tive medicine. J Cell Sci. 2008 Feb 1;121(Pt 3): 255-64.
7. Umehara H, Kimura T, Ohtsuka S, Nakamura T, Kitajima K, Ikawa M, et al. Efficient deriva-tion of embryonic stem cells by inhibition of glycogen synthase kinase-3. Stem Cells 2007 Nov;25(11):2705-11.
8. Leblond CP. Classification of cell populations on the basis of their proliferative behavior. Natl Cancer Inst Monogr. 1964 Feb;14(1):119–50.
9. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Mini-mal criteria for defining multipotentmesenchy-mal stromal cells. The Interna Soc for cell therap pos state. Cytother. 2006 Apr;8(4):315-7.
10. Izadpanah R, Trygg C, Patel B. Biologic Properties of msenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem. 2006 Dec 1;99(5):1285-97.
11. Kern S, Eichler H, Stoeve J. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose Tis-sue. Stem Cells 2006 May;24(5):1294-301.
12. Pountos I, Jones E, Tzioupis C, McGonagle D, Giannoudis PV. Growing bone and cartilage. The role of mesenchymal stem cells. J Bone Joint Surg Br. 2006 Apr;88(4):421-6.
13. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002 Aug;81(8):531-5.
14. Cordeiro MM. Dental pulp tissue engineer-ing with stem cells from exfoliated deciduous teeth. J Eng. 2008 Dec; 34(6):962-9.
15. Huamg ET. Stem progenitor cell mediated de novo regeneration of dental pulp with newly deposited contiuous layer of dentine in an in vivo model .Tissue Eng. part A 2010 Feb;16(2):605-15.
16. Rimondini L, Mele S. Stem cell technology for tissue regeneration in dentistry. Minerva Stomatol. 2009 Oct; 58(10):483-500.
17. Honda MJ, Fong H, Iwatsuki S, Sumita Y, Sarikaya M. Tooth forming potential in embry-onic and postnatal tooth bud cells. Med Mol Morphol. 2008 Dec;41(4):183-92.
18. Yamamoto H, Kim EJ, Cho SW, Jung HS. Analysis of tooth formation by reaggregated dental mesenchyme from mouse embryo. J Electron Microsc. (Tokyo). 2003 Dec;52(6):559-66.
19. Shinumura T. Quiensent epithelial cells rest of malassez differentiate into ameloblast like cells. J Cell Physio. 2008 Dec;217(3):728-38.
20. Melcher AH. On the repair potential of peri-odontal tissues. J Periodontol. 1976 May; 47(5): 256-60.
21. Ballas CB, Zielske SP, Gerson SL. Adult bone marrow stem cells for cell and gene thera-pies: implications for greater use. J Cell Bio-chem. Suppl 2002;38:20-8.
22. Luan X, Dangaria S, Ito Y, Walker C, Jin T, Schmidt M. Neural crest lineage segregation: A blueprint periodontal regeneration. J Dent Res. 2009 Sep; 88(9):781-91.
23. Yokoi T, Saito M, Kiyono T, Iseki S, Kosa-ka K, Nishida E, et al. Establishment of immor-talized dental follicle cells for generating perio-dontal ligament in vivo. Cell Tissue Res. 2007 Feb; 327(2):301-11.
24. Dogan A, Ozdemir A, Kubar A, Oygur T. Healing of artificial fenestration defects by seeding of fibroblast-like cells derived from regenerated periodontal ligament in a dog: a pre-liminary study. Tissue Eng. 2003 Dec;9(6):1189-96.
25. Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing and regeneration. Crit Rev Oral Biol Med. 2002 Dec;13(6):474-84.
26. Wikesjo UM, Xiropaidis AV, Thomson RC, Cook AD, Selvig KA, Hardwick WR. Periodon-tal repair in dogs: rhBMP-2 significantly en-hances bone formation under provisions for guided tissue regeneration. J Clin Periodontol. 2003 Aug;30(8):705-14.
27. Gay IC, Chen S, MacDougall M. Isolation and characterization of multipotent human peri-odontal ligament stem cells. Orthod Craniofac Res. 2007 Aug;10(3):149-60.
28. Kémoun P, Gronthos S, Snead ML, Rue J, Courtois B, Vaysse F, Salles JP, Brunel G. The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro. Biomaterials 2011 Oct;32(30):7375-88.
29. Seo B M, Miura M, Gronthos S, Bartold P M, Batouli S, Brahim J, Young M, Robey P G, Wang C Y, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004 Jul 10-16; 364 (9429):149-55.
30. Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A. Investigating a clonal hu-man periodontal ligament progenitor/stem cell line in vitro and in vivo. J Cell Physiol. 2008 Jun;215(3):743-9.
31. Ma Z, Li S, Song Y, Tang L, Ma D, Liu B, Jin Y. The biological effect of dentin non colla-genous proteins (DNCPs) on the human perio-dontal ligament stem cells (HPDLSCs) in vitro and in vivo. Tissue Eng Part A. 2008 Dec;14 (12):2059-68.
32. Chang XM, Liu HW, Jin Y, Liu Y, He HX. Isolation and identification of dog periodontal ligament stem cells. Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Feb;27(1):79-83.
33. Kemoun P, Laurencin-Dalicieux S, Rue J, Farges JC, Gennero I, Conte-Auriol F, et al. Human dental follicle cells acquire cemento-blast features under stimulation by BMP-2/-7 and enamel matrix derivatives (EMD) in vitro. Cell Tissue Res. 2007 Aug;329(2):283-94.
34. Morsczeck C. Gene expression of runx2, Osterix, c-fos, DLX-3, DLX-5, and MSX-2 in dental follicle cells during osteogenic differenti-ation in vitro. Calcif Tissue Int. 2006 Feb; 78 (2):98-102.
35. Yang Y, Rossi F, Putnins E. Periodontal re-generation using engineered bone marrow mes-enchymal stromal cells. Biomaterials. 2010 Nov;31(33):8574-82.
36. Kawaguchi H, Hirachi A , Hasegawa N , Iwata T. Enhancement of periodontal tissue re-generation by transplantation of bone marrow mesenchymal stem cells. J periodontal. 2004 Sep;75(9):1281-7.
37. 37- Hasegawa N, Kawaguchi H, Hirachi A, Takeda K, Mizuno N, Nishimura M, et al. Be-havior of transplanted bone marrow-derived mesen-chymal stem cells in periodontal defects. J Peri-odontol. 2006 Jun;77(6):1003-7.
38. Liu Y, Zheng Y, Ding G, Fang D, Zhang C, Bartold P M, et al. Periodontal ligament stem cell-mediated treatment for periodontitis in min-iature swine. Stem Cells 2008 Apr;26(4):1065-73.
39. Yang Z, Jin F, Zhang X, Ma D, Han C, Huo N, et al. Tissue engineering of cementum/ peri-odontal-ligament complex using a novel three-dimensional pellet cultivation system for human periodontal ligament stem cells. Tissue Eng Part C Methods. 2009 Dec;15(4):571-81.
40. Buser D, Gallucci GO, Lin Y, Bosshardt D, Belser UC, Yelick PC. Bioengineered periodon-tal tissue formed on titanium dental implants. J Dent Res. 2011 Feb;90(2):251-6.
41. Park JY, Jeon SH, Choung PH. Efficacy of periodontal stem cell transplantation in the treatment of advanced periodontitis. Cell Trans-plantation. 2011; 20(2):271-85.
42. Lin NH, Gronthos S, Bartold PM. Stem cells and periodontal regeneration. Aust Dent J. 2008; 53:108-21.
43. Duan X, Tu Q, Zhang J, Ye J, Sommer C, Mostoslavsky G. Application of induced Plu-ripotent Stem (iPS) Cells in Periodontal Tissue Regeneration. J Cell Physiol. 2011 Jan;226(1): 150-7.
44. Feng F, Akiyama K, Liu Y, Yamaza T, Wang T-M, Chen j-H, et al. Utility of PDL pro-genitors for in vivo tissue regeneration: a report of 3 cases. J Oral Dis. 2010 Jan;16(1):20-8.

XML     Print

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 3 (7-2012) Back to browse issues page