Comparison of Candidal and Bacterial Adherence to Denture Base Acrylic Resins Dr. Ebrahimi Saravi M.,1 Dr. Zomorodian K .,2 Dr. Vojdani M ., 3 Dr. Sattari M.4 1 Assistant Professor, Department of Prosthodontic, School of Dentistry, Mazandaran University of Medical Sciences. Mazandaran, Iran . 2 Assistant Professor, Department of Mycology & Parasitology, School of Medicine, Shiraz University of Medical Sciences. Shiraz, Iran. 3 Associate Professor, Biomaterial Research Center, School of Dentistry, Shiraz University of Medical Sciences. Shiraz, Iran . 4 Associate Professor, Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences. Tehran, Iran . (Received 19 May, 2012 Accepted 18 Oct, 2012) Abstract Background and Aim : Futura Gen and GC Reline Hard denture chairside reline materialshave recently been introduced to the dental market. Although yet to be scientifically proven, these products are claimed to have optimal characteristics. This study aimed to compare the adherence of Candida strains and bacteria to denture base acrylic resins . Materials and Methods : In this in-vitro experimental study, 24 discs were fabricated of Futura Gen, GC Reline Hard and Meliodent reline acrylic resins. Specimens were inoculatedwith three Candida strains and Streptococcus mutans at three time points and after staining,their optical absorbance (microbial adhesion and biofilm formation) was calculated using spectrophotometry. Two-way ANOVA and Tukey’s HSD test were applied for statistical analysis . Results : Absorbance (optical density)of C. albicans strains on Futura Gen, GC Reline and Meliodent hard reline acrylic resins was 0.022, 0.011 and 0.028 at one hour, 0.057, 0.022 and 0.062 at 24 hours and 0.101, 0.051 and 0.11 at one week, respectively. Absorbance of C. glabrata on the mentioned resins was 0.012, 0.008 and 0.016 at one hour, 0.039, 0.029 and 0.044 at 24 hours and 0.075, 0.068 and 0.081 at one week, respectively. Absorbance of C. dubliniensis on these resins was 0.026, 0.035 and 0.027 at one hour, 0.064, 0.066 and 0.067 at 24 hours and 0.11, 0.12 and 0.13 at one week, respectively. Absorbance of standard strain of S. mutans was 0.027, 0.014 and 0.035 at one hour, 0.064, 0.026 and 0.064 at 24 hours and 0.11, 0.05 and 0.11 at one week, respectively. Candidal and bacterial adhesion to denture base acrylic resins was not significantly different at the understudy time points (P>0.05 ). Conclusion : Except for C. dubliniensis, the lowest absorbance belonged to Meliodent. However, absorbance increased over time. Optical absorbance of S. mutans was lower in Meliodent. Among yeast strains, the highest absorbance belonged to C. dubliniensis . Key words: Absorbance , adhesion , candida albicans , streptococcus mutans , acrylic resin Corresponding Author: Dr. Vojdani M . , Associate Professor, Biomaterial Research Center, School of Dentistry, Shiraz University of Medical Sciences. Shiraz, Iran. e.mail: vojdanim@yahoo.com |
