Comparison of the Substantivity of Several Mouthwashesand their Effect on Microbial Plaque using Epifluorescence Microscope

Ghasemi , Mahmood; Rahbar , Mohammad; Valaei , Naser

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Volume 26, Issue 2 (4-2014)

J Iran Dent Assoc 2014, 26(2): 122-128

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Comparison of the Substantivity of Several Mouthwashesand their Effect on Microbial Plaque using Epifluorescence Microscope

Mahmood Ghasemi ^*

¹, Mohammad Rahbar²

, Naser Valaei³

1- Associate Professor and Head, Department of Periodontics, School of Dentistry, Islamic Azad University. Tehran, Iran , mahiran99@yahoo.com
2- Associate Professor, Department of Microbiology, School of Dentistry, Islamic Azad University. Tehran, Iran
3- Member of Scientific Staff of Thalasemia Research Center, Mazandaran University of Medical Sciences. Mazandaran, Iran

Abstract: (9282 Views)

Background and Aim : Several techniques have been used to assess the efficacy and substantivity of mouthwashes. Considering the variability of available mouth rinses, this study aimed to assess the substantivity of several mouthwashes and their effect on microbial plaque using an epifluorescence microscope .

Materials and Methods : This crossover double blind clinical trial evaluated 0.2%, and 0.12% (from 2 different brands) chlorhexidine (CHX), Persica and cetyl pyridinium chloride (CPC) mouth washes as well as normal saline as the negative control. Non-stimulated saliva samples were obtained from 16 candidates at baseline, 30s, 1, 3, 5 and 7h after one time use of mouthwashes. Epifluorescence technique was used to assess the viability of bacteria. For clinical examination following prophylaxis, subjects were asked not to use any oral hygiene measure except for the provided mouthwashes (twice a day) for 4 days. A 10-day washout period was allowed between the use of mouthwashes. Repeated measures ANOVA and Scheffe's test were applied for the comparison of viable bacterial count between the groups and Kruskal Wallis test was used for the assessment of microbial plaque .

Results : Persica and 0.12% CHX maintained their substantivity for 3 and 5h, respectively. A significant reduction in bacterial count was observed up to 7h after the use of 0.2% CHX only (P<0.001). On clinical examination, 0.2% and 0.12% CHX mouthwashes had significant differences with the others but had no significant difference with one another (P<0.02). Persica and CPC had similar efficacy (P<0.02 ).

Conclusion : Efficacy of mouthwashes strongly depends on their substantivity. Daily application frequency of other mouth rinses should be increased in order to achieve an efficacy equal to that of CHX .

Keywords: Chlorhexidine, Cetyl pyridinium chloride, Saliva, Epifluorescence microscope, Substantivity

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Type of Study: Original | Subject: Periodontology

References

1. Addy M. Chlorhexidine compared with other locally delivered antimicrobials. A short review. J Clin Periodontal. 1986 Nov; 13(10):957-64.

2. Addy M, Moran JM. Clinical indications for the use of chemical adjuncts to plaque control: Chlorhexidine formulations. Periodontol 2000, 1997 Oct; 15(1):52-54.

3. Loe H, Schiott CR. The effect of mouthrinses and topical application of chlorhexidine on the development of dental plaque and gingivitis in man. J Periodontal Res. 1970 Apr; 5(2):79-83.

4. Agerbaek N, Melsen B, Rölla G. Application of chlorhexidine by oral irrigation systems. Scand J Dent Res. 1975 Sept; 83(5):284-287.

5. Elworthy A, Greenman J, Doherty FM, New-combe RG, Addy M. The substantivity of a number of oral hygiene products determined by the duration of effects on salivary bacteria. J Periodontol. 1996 Jun; 67(6):572-576.

6. Poosti M, Radvar M, Yaghoobi S, Ahmadi R. Comparing the effect of Chlorhexidine and Per-sica mouthrinses on periodontal status of fixed orthodontic patients. J Mashad Dent Sch. 2006 Winter; 30(3-4):183-190.

7. Mandel I D. Chemotherapuetic agents for con-trolling plaque and gingivitis. J Clin Periodontal. 1988 Sept; 15(8):488-498.

8. Hull PS. Chemical inhibition of plaque. J Clin Periodontol. 1980 Dec; 7(6):431-442.

9. Berchier CE, Slot DE, Van der Weijden GA. The efficacy of 0.12% chlorhexidine mouth-rinse compared with 0.2% on plaque accumulation and periodontal parameters: A systematic re-view. J Clin Periodontol. 2010 Sept; 37(9):829-839.

10. Versteeg PA, Rosema NAM, Hoenderdos NL, Slot DE, Van der weijden GA. The plaque in-hibitory effect of a CPC Mouth rinse in a 3-day plaque accumulation model- a cross- over study. Int J Dent Hyg. 2010 Nov; 8(4):269-275.

11. Seyedein M, Shafiei S. Comparing the persica and chlorhexidine mouthrinses in the treatment of gingivitis. [Thesis], Tehran: Dental School Shahid Beheshti University of Medical Siences; 1996.

12. Tomás I, Cousido MC, Tomás M, Limeres J, García-Caballero L, Diz P. In vivo bactericidal effect of 0.2% chlorhexidine but not 0.12% on salivary obligate anaerobes. Arch Oral Biol. 2008 Dec;53(12):1186-1191.

13. Tomás I, García-Caballero L, Cousido MC, Li-meres J, Alvarez M, Diz P. Evaluation of chlor-hexidine substantivity on salivary flora by Epifluorescence microscopy. Oral Dis. 2009 Sept; 15(6):428-433.

14. Turesky S, Gilmore ND, Glickman I. Reduced plaque formation by the chloromethyl analogue of victamine C. J Periodontol. 1970 Jan; 41(1):41-43.

15. Loe H, Silness J. Periodontal disease in preg-nancy.1. Prevalence and severity. Acta Odontol Scand. 1963 Dec; 21(6):533-551.

16. Jenkins S, Addy M, Newcombe RG. Dose re-sponse of chlorhexidine against plaque and comparison with triclosan. J Clin Periodontol. 1994 Apr; 21(4):250-255.

17. Keijser JA, Verkade H, Timmerman MF, Van der Weijden FA. Comparison of 2 commersially available chlorhexidine mouthrinses. J Perio-dontol. 2003 Feb; 74(2):214-218.

18. Van Strydonck DA, Timmerman MF, van der Velden U, van der Weijden GA. Plaque inhibi-tion of two commercially available chlorhexi-dine mouthrinses. J Clin Periodontol. 2005 Mar; 32(3): 305-309.

19. García-Caballero L, Carmona IT, González MC, Posse JL, Taboada JL, Dios PD. Evaluation of the substantivity in saliva of different forms of application of chlorhexidine. Quintessence Int. 2009 Feb; 40(2):141-144.

20. Cousido MC, Tomás Carmona I, García-Caballero L, Limeres J, Alvarez M, Diz P. In vivo substantivity of 0.12% and 0.2% chlorhex-idine mouthrinses on salivary bacteria. Clin Oral In-vestig. 2010 Aug;14(4):397-402.

21. Harper PR, Milsom S, Wade W, Addy M, Mo-ran J, Newcombe RG. An approach to efficacy screening of mouthrinses: studies on a group of French products (II). Inhibition of salivary bac-teria and plaque in vivo. J Clin Periodontol. 1995 Sep; 22(9):723-727.

22. Quirynen M, Avontroodt P, Peeters W, Pauwels M, Coucke W, van Steenberghe D. Effect of different chlorhexidine formulations in mouth-rinses on de novo plaque formation. J Clin Per-iodontol. 2001 Dec; 28(12):1127-1136.

23. Pizzo G, Guiglia R, Imburgia M, Pizzo I, D'An-gelo M, Giuliana G. The effects of antimicrobial sprays and mouthrinses on supragingival plaque regrowth: a comparative study. J Periodontol. 2006 Feb;77(2):248-256.

24. Lotufo R, Calil CM, Feng HS, Sekiguchi RT, Stewart B, DeVizio W, Proskin HM. Clinical investigation of the efficacy of a commercial mouthrinse containing 0.05% cetylpyridinium chloride in preventing dental plaque. J Clin Dent. 2009 Jun; 20(2):50-54.

25. Hernandez-Cott PL, Elias Boneta A, Stewart B, DeVizio W, Proskin HM. Clinical investigation of the efficacy of a commercial mouthrinse containing 0.05% cetylpyridinium chloride in reducing dental plaque. J Clin Dent. 2009 Jun; 20 (2):39-44.

26. Gjermo P. Chlorhexidine and related substanc-es. J Dent Res. 1989 Nov; 68(11): Special Issue: 1602-1608.

27. Bonesvoll P, Gjermo P. A comparision between chlorhexidine and some quaternary ammonium compounds with regard to retention, salivary concentration and plaque-inhibiting effect in the human mouth after mouth rinses. Arch Oral Bi-ol. 1978 Feb; 23(4):289-294.

28. Pan PC, Harper S, Ricci-Nittel D, Lux R, Shi W. In-vitro evidence for efficacy of antimicrobial mouthrinses. J Dent. 2010 Jun;38 Suppl 1:S16-20.

29. Khoursand A, Salehisormaghi MH, Salehifard R. Clinical comparison on the effect of persica and chlorhexidine mouthwashes on pockets with (4-5) mm depth at the first stage of perio-dontal treatment. J Islamic Dent Ass. 2004 Spring; 16(1):33-39.

30. Moeintaghavi A., Ebrahimnezhad ZA. Clinical evaluation of the effect of persica and 0.2% chlorhexidine mouthwashes on plaque and gin-givitis. J Dent Sch. Shaihid Beheshti University of Medical Siences. 2002 Fall; 20(3):370-378.

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Ghasemi M, Rahbar M, Valaei N. Comparison of the Substantivity of Several Mouthwashesand their Effect on Microbial Plaque using Epifluorescence Microscope. J Iran Dent Assoc 2014; 26 (2) :122-128
URL: http://jida.ir/article-1-1603-en.html

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Volume 26, Issue 2 (4-2014)

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