Effect of locally delivered xanthan-based CHLO-SITE gel along with scaling and root planning on reduction of gingival pocket microbial counts

Dr. Abrishami MR.,¹ Dr. Iramloo B.,² Dr. Eslami G.,³ Dr. Akbarzadeh-Bagheban A.⁴

1Assistant Professor, Department of Periodontics, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences. Tehran, Iran. 2Professor, Department of Periodontics. Faculty of Dentistry, Shahid Beheshti University of Medical Sciences. Tehran, Iran. 3Assistant Professor, Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences. Tehran, Iran. 4Assistant Professor, Department of Biostatistics and member of Dental Research Center, Paramedical Faculty, Shahid Beheshti University of Medical Sciences. Tehran, Iran.

(Received 25 Oct, 2007 Accepted 7 July, 2008)

Abstract

Background and Aim: The locally delivered antibiotics are used as adjunct therapy together with scaling and root planning for periodontitis conditions. The aim of this study was to evaluate the microbiological effectiveness of locally delivered xanthan-based CHLO-SITE gel as an addition to scaling and root planning in treatment of cases with moderate to advanced chronic periodontitis.

Materials and Methods: A randomized controlled split-mouth clinical trial method was used in this investigation. A total of 20 subjects were recruited from patients referring to dental clinics at the School of Dentistry, Shaheed Beheshti University of Medical Sciences. These cases were all met by the study’s inclusion criteria. Participation was voluntary and consent forms were obtained prior to study initiation. One quadrant with pocket depth of 4-6 mm was assigned as the experimental site and the opposite quadrant with similar condition selected as control randomly. After scaling and root planning on both sides, the CHLO-SITE, a xanthan-based gel was injected into the gingiva of the experimental sites. The pocket samples were taken from both sides at baseline, 1 and 3 months after treatment initiation. The samples were exposed to MacConkey, blood agar and nutrient agar mediums and colony counts (CC) were determined. The data were analyzed using Wilcoxon Signed Rank and Friedman tests.

Results: At baseline, the CC of the experimental and control sites were 2690000 and 2645000 respectively with no significant difference. At 1 month follow up, the CC was 13850 and 151610 at experimental and control sites respectively with a significant difference (p<0.027). The CC was 13400 at experimental sites, while it was 143555 at control sites after 3 months follow up. The difference between the two groups was statistically significant as well (p<0.05). This observation showed intensive CC reduction during the first follow up period, while the decrease was slightly less after 3 months follow up.

Conclusion: Based on the results of this investigation, it was revealed that, subgingival injection of xanthan-based CHLO-SITE gel after scaling and root planning in patients with chronic periodontitis, may cause significant decrease in the colony counts compared with scaling and root planning alone.

Keywords: p53 protein - ki67 antigen - Oral leukoplakia - Immunohistochemistry - Staining.

Corresponding Author: Dr. Abrishami MR., Assistant Professor, Department of Periodontics, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences. Tehran, Iran.

e.mail: sh_adhami@kmu.ac.ir