Evaluation of coral (madrepora) cytotoxicity in fibroblast cell culture
Dr. Azimi HR.,1 Dr. Ghazanfari T.2
1 Assistant Professor, Oral & Maxillofacial Department, Faculty of Dentistry, Shahed University. Tehran, Iran. 2 Associate Professor, Department of immunology, Faculty of Medicine, Shahed University. Tehran, Iran.
(Received 25 Nov, 2007 Accepted 25 Nov, 2008)
Background & Aim: Bone defect is one of the major problems facing dentists and maxillofacial surgeons. Several approaches, such as incorporating autografts, xenografts, as well as using polymers have been suggested. The calcified skeleton of corals has been proposed for bone grafting over the past few years. Toxicity is the most important concern of grafting foreign material. The purpose of this study was to investigate the toxicity of coral (madrepora) skeleton in fibroblast culture.
Materials & Methods: Powdered corals with particle sizes of less than 100 micron were autoclaved. Coral powder was added to culture medium consisting of 90% RPMI, 10% FBS and different concentrations of 0.5, 1, 2, 5, 10, 20 and 50 milligrams per 100 milliliters of media were prepared. Fibroblast cells were obtained from Pasteur institute for inserting into culture mediums. Three samples were prepared from each concentration. Complete medium culture was the control. Ninety six well plate containing culture medium, fibroblasts and coral powder were prepared. They were placed into a programmed incubator containing 5% CO2 for 24, 48 and 72 hours at 37○C. The (MTT) was then added to plate culture mediums. Since light absorption has close relationship with cells viability, ELISA reader apparatus was used to measure light absorption (optic dosimeter). The collected data was statistically analyzed using ANOVA test.
Results: Light absorption in control group was similar to light absorption in the various concentrations of coral medium culture along with incorporated fibroblasts.
Conclusion: Based on the findings of this study, it can be concluded that coral (madrepora) has potentially no cytotoxic effect on fibroblast cells.
Key words: Coral - Fibroblast – Cytotoxicity.
Corresponding Author: Dr. Azimi HR., Assistant Professor, Oral & Maxillofacial Department, Faculty of Dentistry, Shahed University. Tehran, Iran.