TY - JOUR JF - JIDA JO - J Iran Dent Assoc VL - 17 IS - 5 PY - 2005 Y1 - 2005/8/01 TI - Antibacterial and substantivity evaluation of 2.5% sodium hypochlorite, 0.2% cholorhexidine and distilled water as root canal irrigants (In - vitro) TT - ارزیابی اثر ضدباکتریایی و دوام اثر هیپوکلریت سدیم 5/2%، کلرهگزیدین 2/0% و آب مقطر جهت شستشوی کانال (In vitro) N2 - Antibacterial and substantivity evaluation of 2.5% sodium hypochlorite, 0.2% cholorhexidine and distilled water as root canal irrigants (In - vitro) Dr. S.M. Hasheminiya* - Dr. S.A. Havaee** - Dr. M. Rajabi*** * - Assistant Professor of Endodontics Dept. - Faculty of Dentistry – Isfahan University of Medical Sciences. **- Assistant Professor of Microbiology Dept. - Faculty of Medicine – Isfahan University of Medical Sciences.***- Dentist. Background and Aim: The use of an irrigating solution during root canal cleaning and shaping is necessary for the elimination of all irritants. The aim of this study was to compare the anti-bacterial efficacy of distilled water, chlorhexidine 0.2% and sodium hypochlorite 2.5% as root canal irrigants on the reduction of Entrococcus fecalis (E.fecalis)colonies in extracted human teeth. Materials and Methods: In this experimental study, 80 extracted human teeth with single ca-nal were selected. After preparation all root canals were contaminated with E. fecalis. Then, filing was done up to file ≠40 and flaring up to ≠70. During filing and flaring procedures irri-gation was done using 20 cc of each solution. Finally, paper points were used to obtain sam-ples which were then sent to laboratory for CFU (colony – forming unit) count after incuba-tion. Sampling was done once after placing teeth in the incubator for one week. Data were sub-jected to Kruskal – Wallis and Duncan tests. Results: The finding of this study showed that sodium hypochlorite and chlorhexidine were more effective in the reduction of microorganisms' counts than distilled water (PV SP - 38 EP - 45 AU - Hasheminiya, S.Mohsen AU - Havaee, S.Asghar AU - Rajabi, Mahmood AD - KW - Chlorhexidine KW - Hypochlorite KW - Root canal irrigant KW - Substantivity KW - Colony KW - forming unit (CFU) KW - E. fecalis UR - http://jida.ir/article-1-147-en.html ER -