Iranian Dental Association Journal of Iranian Dental Association 3060-8201 21 2 2009 09 01 Antimicrobial effects of sanosil solution on dental instruments 150 155 EN Jamileh Taheri Fahimeh Anbari Negin Katebi Fatemeh Fallah Mohammadjavad Kharazifard Journal Article 2009 09 14 2013 02 25  Antimicrobial effects of sanosil solution on dental instruments    <u> Dr. Taheri JB </u><u>., </u>1 Dr. Anbari F ., 2 Dr. Katebi N ., 3 Dr. Fallah F . 4 Dr. Kharazifard MJ .5  1 Associate Professor, Department of Oral Medicine, School of Dentistry, Shahid Beheshti University / MC. Tehran, Iran. 2 Resident, Specialy Program, Department of Oral Medicine, School of Denistry, Shahid Beheshti University / MC. Tehran, Iran. <font size="2">3 </font><font size="2">Dentist. 4 Associate Professor, Department of Microbiology, School of Medicine Shahid Beheshti University / MC. Tehran, Iran. 5 Dentist, Member Dental Research Center, Tehran University / Medical Sciences. Tehran. Iran.</font>   <i> (Received 3 July, 2008 Accepted 23 July, 2009) </i>   <i> Abstract </i>   <i> Background and Aim </i>: <i>Sanosil </i>is a brand new disinfectant that is composed of H2O2 and traces of Ag with considerable effectiveness. The aim of this study was to evaluate the effectiveness of <i>Sanosil</i> on disinfecting dental devices and instruments.  <i> Materials </i><i>and </i><i>Methods </i>: This laboratory-based study was conducted on 60 samples that were collected before (n=30) and after (n=30) administration of <i>Sanosil</i> from the same spots on dental instruments. The samples were cultured on blood agar and chocolate agar and were incubated in 37 â—‹ C for 24 hours. The effect of <i>Sanosil</i> was investigated by counting the number of microbial colonies after determining the standard laboratory species’ type and subtype. The data were analyzed by using SPSS version 14 and Chi-square test for determining the significance level, and alpha error was set at 0.05.  <i> Results </i>: The isolated microorganisms were mainly: Staphylococcus aureus, Streptococcus viridans, coagolase negative Staphylococcus, E.coli, Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis, E.coli Aeromonas hydrophila, corynebacterium, Microccus luteus. From the total samples 90% were free of any bacteria. Microbial load was significantly reduced (P<0.05) after <i>Sanosil</i> application on dental instruments and it also deferred the growth of standard microorganisms in vitro.  <i> Conclusion </i>: <i><i>Sanosil </i>is an effective agent in decreasing bacterial contamination of dental instruments without any adverse effect on the surface of instruments. </i> <i> Key words </i>: Disinfection - Microbial culture – <i>Sanosil </i>- Dental instruments.  <i> </i> <i> </i> <i> Corresponding Author </i>: <i><i>Dr. </i><i>Taheri JB., <i>Associate Professor, Department of Oral Medicine, School</i> <i>of Dentistry, Shahid Beheshti University / MC. Tehran, Iran. </i></i></i> <i> e.mail: </i><i><i>JM_TAHERI2006@yahoo.com </i><i></i></i>Â