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:: Volume 27, Issue 4 (10-2015) ::
J Iran Dent Assoc 2015, 27(4): 203-208 Back to browse issues page
A Comparative Study of Immunohistochemical Expression of BAX Protein in Radicular Cyst and Keratocystic Odontogenic Tumor
Shima Nafarzadeh1, Ahmad Reza Sarrafan *2, Hamid Abbaszadeh Bidokhti3, Hemat Gholinia4
1- Assistant Professor, Department of Oral and Maxillofacial Pathology, School of Dentistry, Babul University of Medical Sciences, Babul, Iran
2- Dental Student, Member of Research Committee, School of Dentistry, Babul University of Medical Sciences, Babul, Iran , a.sarrafan@mubabol.ac.ir
3- Member of Dental Material Research Center, School of Dentistry, Babul University of Medical Sciences, Babul, Iran
4- Health Research Institute, Babul University of Medical Sciences, Babul, Iran
Abstract:   (2483 Views)

Background and Aim: Keratocystic odontogenic tumor (KCOT), formerly known as odontogenic keratocyst (OKC), is a developmental odontogenic cyst with an invasivebehavior and high recurrence rate. Radicular cyst (RC) is an inflammatory non-invasive dental cyst, with no tendency to recurrence. Bcl2-associated X (BAX) protein acts as an apoptotic activator (pro-apoptotic protein), disrupts the integrity of mitochondrial membrane, and leads to the loss of outer membrane and release of cytochrome c from the inner membrane into the cytosol. Since KCOT is more aggressive and has a greater tendency to recurrence than RC, the aim of this study was to assess the immunohistochemical expression of BAX protein in the epithelial lining of RC and KCOT.

Materials and Methods: In this descriptive analytical study, paraffinized blocks of 17 RCs and 17 KCOTs fixed in 10% formalin were used (all KCOT samples were non-inflammatory and non-syndromic). After confirming the diagnoses by reviewing the slides, tissuespecimens with sufficient length of epithelium were stained by immunohistochemistry (IHC) for BAX marker. Then, the stained cells were randomly counted in five high power fields. Analysis of the data was performed using SPSS 21 via Student's t-test and chi-test.

Results: Percentages of staining for BAX marker were 16.4±9.4% and 66.4±12.3% in the epithelial lining of KCOT and RC, respectively and there was a significant difference between them (P<0.001).

Conclusion: According to the findings, lower expression of BAX protein in KCOT justifies its more aggressive behavior and tendency to recurrence compared to RC.

Keywords: BAX protein, human, Odontogenic Cysts, Radicular Cyst, Immunohistochemistry
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Type of Study: Original | Subject: Pathology
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Nafarzadeh S, Sarrafan A R, Abbaszadeh Bidokhti H, Gholinia H. A Comparative Study of Immunohistochemical Expression of BAX Protein in Radicular Cyst and Keratocystic Odontogenic Tumor. J Iran Dent Assoc 2015; 27 (4) :203-208
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Volume 27, Issue 4 (10-2015) Back to browse issues page
Journal of Iranian Dental Association


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