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:: Volume 27, Issue 2 (4-2015) ::
J Iran Dent Assoc 2015, 27(2): 85-89 Back to browse issues page
Efficacy of Four Disinfectants Against Hepatitis B Virus
Mohammad reza Aghasadeghi1 , Sakine Arami2 , Masomeh Hasani Tabatabaie3 , Hojjat Darvishpour * 4, Mohammad javad Kharazifard5 , Hamid Sarlak6
1- Associate Professor, Department of Virology, Pasteur Institute of Iran, Tehran, Iran
2- Assistant Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences AND Department of Operative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
3- Associate Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences AND Department of Operative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
4- Postgraduate Student, Department of Orthodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
5- Research Member of Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
6- Postgraduate Student, Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (4369 Views)

Background and Aim: Hepatitis B virus (HBV) is an important infectious agent in denti-stry necessitating the use of disinfectants to prevent its transmission. This study compared the efficacy of 2/100 sodium hypochlorite disinfectants prepared from 5% solution manufactured by four different manufacturers namely Ashimashi, Paknaz, Vitex and Active for disinfection of surfaces infected with HBV.

Materials and Methods: In this in vitro experimental study, sera of 10 hepatitis B pa-tients were poured into microtubes and 2/100 sodium hypochlorite solutions prepared from 5% Ashimashi, Paknaz, Vitex and Active disinfectants were added to them in 1/1 ratio. Polymerase chain reaction (PCR) was performed using virus diagnostic kits to detect the viral genome. Real-time PCR was performed before and after incubation with the disinfectants to assess the viral load in the serum. The reduction in the viral load of HBV was statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests.

Results: No significant antiviral efficacy was noted following the application of Ashima-shi 2/100 sodium hypochlorite disinfectant. Paknaz showed the highest efficacy against HBV. Vitex and Active ranked next with significant differences (P<0.0001)

Conclusion: Under the study limitations, Paknaz 2/100 sodium hypochlorite solution was the most effective while Ashimashi 2/100 sodium hypochlorite disinfectant did not show adequate efficacy against HBV.
Keywords: Hepatitis B, Disinfection, Surface, Dentistry
Full-Text [PDF 136 kb]   (2363 Downloads)    
Type of Study: Original | Subject: General
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Aghasadeghi M R, Arami S, Hasani Tabatabaie M, Darvishpour H, Kharazifard M J, Sarlak H. Efficacy of Four Disinfectants Against Hepatitis B Virus. J Iran Dent Assoc 2015; 27 (2) :85-89
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Volume 27, Issue 2 (4-2015) Back to browse issues page
Journal of Iranian Dental Association

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