@ARTICLE{Hasheminiya, author = {Hasheminiya, S.Mohsen and Havaee, S.Asghar and Rajabi, Mahmood and }, title = {Antibacterial and substantivity evaluation of 2.5% sodium hypochlorite, 0.2% cholorhexidine and distilled water as root canal irrigants (In - vitro)}, volume = {17}, number = {5}, abstract ={Antibacterial and substantivity evaluation of 2.5% sodium hypochlorite, 0.2% cholorhexidine and distilled water as root canal irrigants (In - vitro) Dr. S.M. Hasheminiya* - Dr. S.A. Havaee** - Dr. M. Rajabi*** * - Assistant Professor of Endodontics Dept. - Faculty of Dentistry – Isfahan University of Medical Sciences. **- Assistant Professor of Microbiology Dept. - Faculty of Medicine – Isfahan University of Medical Sciences.***- Dentist. Background and Aim: The use of an irrigating solution during root canal cleaning and shaping is necessary for the elimination of all irritants. The aim of this study was to compare the anti-bacterial efficacy of distilled water, chlorhexidine 0.2% and sodium hypochlorite 2.5% as root canal irrigants on the reduction of Entrococcus fecalis (E.fecalis)colonies in extracted human teeth. Materials and Methods: In this experimental study, 80 extracted human teeth with single ca-nal were selected. After preparation all root canals were contaminated with E. fecalis. Then, filing was done up to file ≠40 and flaring up to ≠70. During filing and flaring procedures irri-gation was done using 20 cc of each solution. Finally, paper points were used to obtain sam-ples which were then sent to laboratory for CFU (colony – forming unit) count after incuba-tion. Sampling was done once after placing teeth in the incubator for one week. Data were sub-jected to Kruskal – Wallis and Duncan tests. Results: The finding of this study showed that sodium hypochlorite and chlorhexidine were more effective in the reduction of microorganisms' counts than distilled water (PV }, URL = {http://jida.ir/article-1-147-en.html}, eprint = {http://jida.ir/article-1-147-en.pdf}, journal = {Journal of Iranian Dental Association}, doi = {}, year = {2005} }