%0 Journal Article %A Hasheminiya, S.Mohsen %A Havaee, S.Asghar %A Rajabi, Mahmood %T Antibacterial and substantivity evaluation of 2.5% sodium hypochlorite, 0.2% cholorhexidine and distilled water as root canal irrigants (In - vitro) %J Journal of Iranian Dental Association %V 17 %N 5 %U http://jida.ir/article-1-147-en.html %R %D 2005 %K Chlorhexidine, Hypochlorite, Root canal irrigant, Substantivity, Colony, forming unit (CFU), E. fecalis, %X Antibacterial and substantivity evaluation of 2.5% sodium hypochlorite, 0.2% cholorhexidine and distilled water as root canal irrigants (In - vitro) Dr. S.M. Hasheminiya* - Dr. S.A. Havaee** - Dr. M. Rajabi*** * - Assistant Professor of Endodontics Dept. - Faculty of Dentistry – Isfahan University of Medical Sciences. **- Assistant Professor of Microbiology Dept. - Faculty of Medicine – Isfahan University of Medical Sciences.***- Dentist. Background and Aim: The use of an irrigating solution during root canal cleaning and shaping is necessary for the elimination of all irritants. The aim of this study was to compare the anti-bacterial efficacy of distilled water, chlorhexidine 0.2% and sodium hypochlorite 2.5% as root canal irrigants on the reduction of Entrococcus fecalis (E.fecalis)colonies in extracted human teeth. Materials and Methods: In this experimental study, 80 extracted human teeth with single ca-nal were selected. After preparation all root canals were contaminated with E. fecalis. Then, filing was done up to file ≠40 and flaring up to ≠70. During filing and flaring procedures irri-gation was done using 20 cc of each solution. Finally, paper points were used to obtain sam-ples which were then sent to laboratory for CFU (colony – forming unit) count after incuba-tion. Sampling was done once after placing teeth in the incubator for one week. Data were sub-jected to Kruskal – Wallis and Duncan tests. Results: The finding of this study showed that sodium hypochlorite and chlorhexidine were more effective in the reduction of microorganisms' counts than distilled water (PV %> http://jida.ir/article-1-147-en.pdf %P 38-45 %& 38 %! %9 Research %L A-10-73-1 %+ %G eng %@ 2383-3041 %[ 2005