Submitted: 13 Sep 2009
Accepted: 25 Feb 2013
ePublished: 25 Feb 2013
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J Iran Dent Assoc. 2009;21(2): 107-114.
  Abstract View: 22

Research

EDTA dentin conditioning and Micro-shear bond strength using SE and SEM

Masomeh Hassani-Tabatabaei, Mostafa Moazzami, Maryam Ghavam, Esmaeil Yassini, Ayoub Pahlavan, Sakineh Arami, Mansoreh Mirzaei, Nayereh Bayat, Hamid Kermanshah*
*Corresponding Author: Email: Kermanshahhamid@yahoo.com

Abstract

 EDTA dentin conditioning and Micro-shear bond strength using SE and SEM

 

 

 Dr. Hassani-Tabatabaei M.,1 Dr. Moazzami SM., 2 Dr. Ghavam M.,1 Dr. Yassini E.,3 Dr. Pahlavan A.,1

 Dr. Arami S.,4 Dr. Mirzaei M.,4 Dr. Bayat N.5 Dr. Kermanshah H .,4

  

  1 Associate Professor, Department of Operative Dentistry, School of Dentistry, Tehran University / Medical Sciences. Tehran, Iran. 2 Associate Professor, Department of Operative Dentistry, School of Dentistry, Mashhad University of Medical Sciences. Mashhad, Iran. 3 Professor, Department of Operative Dentistry, School of Dentistry, Tehran University / Medical Sciences. Tehran , Iran . 4 Assistant Professor, Department of Operative Dentistry, School of Dentistry, Tehran University / Medical Sciences. Tehran, Iran. 5 Resident, Specialty Program, Department of Operative Dentistry, School of Dentistry, Mashhad University of Medical Sciences. Mashhad, Iran.

  

  (Received 14 July, 2008 Accepted 6 May, 2009)

  Abstract

  Background and Aim : The main objective of using adhesive material is the improvement of restorative materials bonding to dentin surface in clean environment. The aim of this study was to investigate the effect of EDTA on bond strength of composite resin to dentin surface when used at different times and concentrations prior to application of adhesive systems by employing Scanning Electronic Microscope (SEM).

  Materials and Method : A total of 100 dentin samples were prepared from 50 human premolars. These samples were divided into two groups according to bonding type ( Clearfil SE Bond =group I, Futurbond NR=group II ) and each one was classified 5 subgroups according to time and concentration of EDTA as follows: 1 – Control. 2- EDTA 15%, 30 seconds. 3 – EDTA 15%, 60 seconds. 4 – EDTA 24%, 30 seconds. 5 – EDTA 24%, 60 seconds. These samples were conditioned and composite cylinders were adhered to them. After 500 cycles of thermocycling, the testing for micro-shear bond strength was conducted on these samples. Another 10 samples were demineralized for 30 seconds with 6NHCL and deproteinized with 2.5% Naocl for 10 minutes, air dried with desiccators and viewed under SEM. Data were analyzed using one-way as well as three-way ANOVA and Tukey tests.

  Results : The mean Micro-shear bond strength in group I (Clearfil SE bond) were: 31.88 ( ± 13) for subgroup 1, 28.4 ( ± 13) in subgroup 2, 29.8 ( ± 9) in subgroup 3, 29.2 ( ± 11) in subgroup 4, and 32.7 ( ± 12) in subgroup 5. The mean Micro-shear bond strength in group 2 (Futurbond NR): were: 22.6 ( ± 10) for subgroup 1, 28.5 ( ± 9) in subgroup 2, 27.3 ( ± 10) subgroup 3, 32.4 ( ± 15) in subgroup 4, and 31.1 ( ± 14) in subgroup 5.

  There were no significant differences between the samples conditioned and the ones not conditioned with EDTA, time and the concentration of EDTA applied and also the two bonding types.

  Conclusions : EDTA conditioning prior to use of the two bonding agents under investigation in this study did not improve the bonding strength of the self etch systems, neither did the time and the concentration of EDTA applied.

  Key words : EDTA - Denting bonding - Self etch - Micro shear bond strength - SEM - Smear layer.

  

  Corresponding Author : Dr. Kermanshah H ., Assistant Professor, Department of Operative Dentistry, School of Dentistry, Tehran University / Medical Sciences. Tehran, Iran .

  e.mail: Kermanshah hamid@yahoo.com

 

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